Abstract
The widespread use of antibiotics has led to an increase in multidrug-resistant bacteria, which represent a serious risk of infection, in view of this problem the aim of this research was performed to characterize the antibacterial effect of ethanolic and petroleum ether extract of Polypodium decumanum leaves against three gram-positive bacteria(Staphylococcus aureus, Bacillus subtilius, and Streptococcus pyogenes) and three gram-negative bacteria(Pseudomonas aeruginosa, Escherichia coli, and Pseudomonas alcaligenes) in vitro by disc-diffusion method. In this study, we have taken 3 concentrations of Polypodium decumanumplant extract (ethanolic and petroleum ether) viz. 50 mg/ml, 100 mg/ml, and 200 mg/ml, and observed that the ethanolic extract of Polypodium decumanumof the concentration of 200 mg/ml showed higher antibacterial effect compare to other concentration against all 3 gram-positive bacteria and all 3 gram-negative bacteria, but not higher than standard drug Ampicillin.
References
Berahou A, Auhmani A, Fdil N, Benharref A, Jana M, and Gadhi CA, Antibacterial activity of Quercus ilex bark's extracts, Journal of Ethnopharmacology,2007;112(3):426-429.[View in PubMed]
Akrayi HFS: Antibacterial Effect of Seed Extract of Cardamom (Elettaria cardamom) against Staphylococcus aureus and Proteus mirabilis. Tikrit J. Pure Sci, 2012;17(2):14-18.
Alzoreky NS, Nakahara K. Antibacterial activity of extracts from some edible plants commonly consumed in Asia. Int J Food Microbiol. 2003; 80:223–30.[View in PubMed]
Thiem B and Go?li?ska O, Antimicrobial activity of Rubuschamaemorus leaves, Fitoterapia,2007;75(1):93-95.[View in PubMed]
Jones FA. Herbs as useful plants: Their role in history and today, European Journal of Gastroenterology & Hepatology, 1996;8(12): 1227-31.
Hammer KA, Carson CF, Riley TV. Antimicrobial activity of essential oils and other plant extracts. J ApplMicrobiol. 1999;86(6):985–990.
Harborne J B, Baxter H. The handbook of natural flavonoids, Vol. 1 and 2. Chichester, UK: John Wiley and Sons; 1999.
Kumar N, Kshrisagar M D, Saini V, GC-MS Analysis of Polypodium decumanum” International Research Journal of Pharmacy,2011; 2(9):155-156.
Lino A, Deogracious O. The in-vitro antibacterial activity of Annona senegalensis, Securidaccalongipendiculataand Steganotaeniaaraliaceae - Ugandan medicinal plants. Afr Health Sci. 2006;6:31–35.
Cowan MM. Plant products as antimicrobial agents, Clinical Microbiology Reviews, 1999;12(4):564-82.[View in Pubmed]
Marjorie MC. Plant products as antimicrobial agents, Clin. Microbiol Rev, 1999; 12(4): 564-582.[View in PubMed]
Lee MH, Kwon HA, Kwon DYet al., Antibacterial activity of medicinal herb extracts against Salmonella, International Journal of Food Microbiology,2006;111(3):270-75.
NCCLS (National Committee for Clinical Laboratory Standards), “Performance standards for antimicrobial disc susceptibility test” 1997, 6th Ed. Approved Standard. M2-A6.
Pathak P,SarsawathyD,Vora A, etal, In vitro antimicrobial activity and phytochemical analysis of the leaves of Annona muricata. Int J Pharma Res Dev.2010; 2:1-6.
Prashanth KV, Chauhan NS, Padh H, Rajani M. Search for antibacterial antifungal agents from selected Indian medicinal plants. J Ethnopharmacol. 2006; 107:182–83.
Ramasamy S, Charles MA. Antibacterial effect of volatile components of selected medicinal plants against human pathogens. Asian J Microbial Biotech Env, 2009;6:209–10.
S. Naz, R. Siddiqi, S. Ahmad, S. A. Rasool, and S. A. Sayeed, Antibacterial activity and isolation of compounds from Punicagranatum, Journal of Food Science,2007;72(9):341-45.
Satish S, RaghavendraM.P., RaveeshaK.A., Evaluation of the Antibacterial potential of some plants against human pathogenic bacteria, Advance in Biological Research, 2008,2:44-48.
Shaik D, Malika FA, Rafi SM, Naqui B. Studies of antibacterial activity of ethanolic extract from Nericumindicum and Hibiscus rosasinensis. J Islamic Acad Sci. 1994; 7:167–8.
Towers GH, Lopez A,Hudson JB, Antiviral and antimicrobial activities of medicinal plants. J Ethnopharmacol. 2001; 77:189-96.[View in PubMed]
Gopala V.Krishnakone and Samy RP. Current status of herbal and their future perspective, Nature Proceedings, 2007,1-13.
WHO, “Regulatory situation of herbal medicine: A World-Wide Review,” World HealthOrganization, 1998, Geneva.
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