EVALUATION OF IMMUNOCHROMATOGRAPHIC ASSAY USED IN SCREENING OF HBsAg AMONG BLOOD DONORS IN A RURAL TEACHING HOSPITAL, SANGAREDDY

Authors

  • Nagababu Pyadala MNR Medical College & Hospital, Sangareddy, T.S, India.
  • Rajaneesh Borugadda MNR Medical College & Hospital, Sangareddy, T.S, India.
  • Revathi Poonati MNR Medical College & Hospital, Sangareddy, T.S, India.
  • Soumendra Nath Maity Malla Reddy Institute of Medical Sciences, Suraram, Hyderabad, India
  • Neve Polavarapu MNR Medical College & Hospital, Sangareddy, T.S, India.
  • Prudhvi Chand Mallepaddi MNR Medical College & Hospital, Sangareddy, T.S, India.
  • Rathnagiri Polavarapu MNR Medical College & Hospital, Sangareddy, T.S, India.

Keywords:

Hepatitis B surface antigen (HBsAg), Immunochromatographic test kit (ICT).

Abstract

Objectives: The present study was to assess ICT kit used in the screening process of HBsAg among blood donors in a rural teaching hospital, sangareddy.

Materials and Methods: In this study 1050 number of blood units were collected from donors containing both voluntary and replacement donors for a period of one year from January 2015 to December 2015. 1050 donors are tested for HBsAg by using ICT kit and ELISA method.

Results: We found 17 out of 1050 subjects tested positive for HBsAg by using ICT kit and ELISA.

Conclusion: The present study concludes that the overall performance of the rapid ICT kit for HBsAg was equally sensitive to ELISA and yet they were cheap and quicker. It can be recommended that ELISA comparable rapid devices may be allowed to be used for initial screening of hepatitis B especially, in remote areas or where cost is an issue.

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References

Lozano R, Naghavi M, Foreman K, Lim S, Shibuya K, Aboyans V, Abraham J, et al. Global and regional mortality from 235 causes of death for 20 age groups in 1990 and 2010: a systematic analysis for the Global Burden of Disease Study 2010. Lancet 2012;380:2095-2128.

Chaudary A. Epidimology of Hepatitis B virus in India.Hep B Annual, 2004; 1:17-24.

WHO core programme clusters. Family and community health. Hepatitis B. Community office for India. (http://www.whoindia.org/en/selection6/section8.htm)

Nagababu Pyadala, Soumendra Nath Maity et al. Seroprevalence of Transfusion Transmissible Infections among Blood Donors attending in a Rural Teaching Hospital at Sangareddy. Int J Biol Med Res.2015;6(4):5249-5253

"Hepatitis B FAQs for the Public". Centers for Disease Control and Prevention. Retrieved 2015-08-24.

Fairley CK, Read TR (February 2012). "Vaccination against sexually transmitted infections". Current Opinion in Infectious Diseases 25 (1): 66–72.

Buddeberg F, Schimmer BB, Spahn DR (September 2008). "Transfusion-transmissible infections and transfusion-related immunomodulation". Best Practice & Research. Clinical Anaesthesiology 22 (3): 503–17.

Guyton and Hall: Textbook of Medical physiology. 11th (ed). Page 640.

Henderson AJW, Carswell F. Circadian rhythm of peak expiratory flow inasthmatic and normal children. Thorax. 1989; 44:410-414.

Rose D, Sudharsanam A, Adankatti T, Babu PG, John TJ,et al. Increasing Prevalence of HIV antibody among blood donors monitored over 9 years in blood donors monitored over 9 years in blood banks. Indian J Med Res. 1998; 108:42–44.

Singh K, Bhat S, Shastry S. Trend in Seroprevalence of Hepatits B virus infection among blood donors of coastal Karnataka, India, J Infect Dev Ctries May 2009; 3(5):376-9.

Arora D, Arora B, Khetarpal A, et al. Seroprevalence of HIV, HBV,HCV and syphilis in blood donors in Southern Haryan. Indian J Pathol Microbiol. 2010; 53:308-319.

Khan JK, Lone DS, Hameed A, Munim MR, Bhatti M, Khattak AA, Usman M, Nadeem MF, Satti HS, Munir M: Evaluation of the performance of two rapid immunochromatographic tests for detection of hepatitis B surface antigen and anti HCV antibodies using ELISA tested samples. Sp Ed Ann 2010, 16:84–87.

Khansari N, Mazhari SZ, Rezvan H: A comparative study of a rapid test and ELISA for the serological determination of HIV infection. Acta Med Iran 1993, 31:68–71.

Qasmi SA, Aqeel S, Ahmed M, Alam SI, Ahmad A. Detection of Hepatitis B Viruses in Normal Individuals of Karachi. J Coll Physicians Surg Pak 2000; 10:467-9.

Kaur H, Dhanao J, Oberoi A. Evaluation of rapid kits for detection of HIV, HBsAg and HCV infections. Indian J Med Sci 2000; 54: 432-4.

Irwig L, Bossuyt P, Glasziou P, Gatsonis C, Lijmer J.Designing studies to ensure that estimates of test accuracy are transferable. BMJ 2002; 324: 669-71.

Published

2016-09-15

How to Cite

Pyadala, N. ., Borugadda, R. ., Poonati, R. ., Maity , S. N., Polavarapu, N. ., Mallepaddi , P. C. ., & Polavarapu , R. . (2016). EVALUATION OF IMMUNOCHROMATOGRAPHIC ASSAY USED IN SCREENING OF HBsAg AMONG BLOOD DONORS IN A RURAL TEACHING HOSPITAL, SANGAREDDY. International Journal of Research and Development in Pharmacy & Life Sciences, 5(5), 2335-2338. Retrieved from https://ijrdpl.com/index.php/ijrdpl/article/view/260

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