HPLC - VALIDATION OF MOXIFLOXACIN
Keywords:Lichrospher RP-18 column, moxifloxacin, MIC
The structure formula of moxifloxacin is described as follows: 8 methoxy flouroquinolone: and it is broad spectrum antibiotic [1, 2].we study moxifloxacin by IV drug delivery system. And for its study following apparatus is used; 1-a reversed-phase Lichrospher RP-18 column, 2-a mobile phase which is composed of different solvents.
It is detected at a wavelength of 290 nm. By this method the retention time of moxifloxacin is very short. When we detect it in plasma and brain tissue linear curves are obtained. .Advantage of this method is that we can detect the bacteria in plasma and brain tissues by this method.
Balfour JAB WL. 1999. Moxifloxacin. Drugs. 363–374
P Mi. 2001. 1125
Farber JM PP. 1991. Listeria monocytogenes. 476-511Microbiological Reviews
Goulet V MP. 1992,1996. Listeriosis in 225 non-pregnant patients Scandinavian Journal of Infectious Diseases 367–374
Grayo S J-LO, Desroches MC, Le Monnier A. 2008. Antimicrobial Agents and Chemotherapy. Comparison of the in vitro efficacies of moxifloxacin and amoxicillin against Listeria monocytogenes 1697–1702.
H H. 2004. Expert Opinion on Pharmacotherapy. An update on the medical management of listeriosis 1727-1735
Grayo S L-DM, Dussurget O, et al. 2008. Antimicrobial Agents and Chemotherapy. Rapid eradication of Listeria monocytogenes by moxifloxacin in a murine model of central nervous system listeriosis 3210–3215
Scortti M L-LL, Wagner M, Chico-Calero I, Losito P, Vázquez-Boland JA. 2006. Coexpression of virulence and fosfomycin susceptibility in Listeria: molecular basis of an antimicrobial in vitro-in vivo paradox. Nature Medicine. 515–517
Ba BB ER, Ducint D, Quentin C, Saux MC. 2001. Determination of moxifloxacin in growth media by high-performance liquid chromatography. Journal of Chromatography 754(1):107–112
Kanellakopoulou K PA, Stroumpoulis K, et al. Pharmacokinetics of moxifloxacin in non-inflamed cerebrospinal fluid of humans: implication for a bactericidal effect. Journal of Antimicrobial Chemotherapy 2008;61(6):1328–1331
Laban-Djurdjevi? A J-SM, Djurdjevi? P. Optimization and validation of the direct HPLC method for the determination of moxifloxacin in plasma. Journal of Chromatography B 2006;844(1):104–111
Liang H KM, Sowinski KM. Separation of levofloxacin, ciprofloxacin, gatifloxacin, moxifloxacin, trovafloxacin and cinoxacin by high-performance liquid chromatography: application to levofloxacin determination in human plasma. Journal of Chromatography B 2002;772(1):53–63
Rodriguez-Cerrato V MC, Michelow IC, et al. Pharmacodynamics and bactericidal activity of moxifloxacin in experimental Escherichia coli meningitis. Antimicrobial Agents and Chemotherapy 2001;45(11):3092–3097
Siefert HM D-BA, Henninger K, Hucke F, Kohlsdorfer C, Stass HH. Pharmacokinetics of the 8-methoxyquinolone, moxifloxacin: a comparison in humans and other mammalian species. Journal of Antimicrobial Chemotherapy 1999;43(supplement B):69–76
Hemanth Kumar AK RG. Simple and rapid liquid chromatography method for determination of moxifloxacin in plasma. Journal of Chromatography B 2009;877(11-12):1205–1208
S TU. High-performance liquid chromatography assay for moxifloxacin: pharmacokinetics in human plasma. Journal of Pharmaceutical and Biomedical Analysis 2007;43(1):320–324
Østergaard C ST, Knudsen JD, Frimodt-Møller N. Evaluation of moxifloxacin, a new 8-methoxyquinolone, for treatment of meningitis caused by a penicillin-resistant pneumococcus in rabbits. . Antimicrobial Agents and Chemotherapy 1998;42(7):1706–1712
Wise R AJ, Marshall G, Hartman G. Pharmacokinetics and inflammatory-fluid penetration of moxifloxacin following oral or intravenous administration. Antimicrobial Agents and Chemotherapy 1999;43(6):1508–1510
Blondiaux N. 2010.Daily serum piperacillin monitoring is advisable in critically ill patients. 35:500–503. [PubMed]. Int. J. Antimicrob. Agents 35:4
Dupuis A. ea. 2001. Pharmacokinetic-pharmacodynamic modeling of electroencephalogram effect of imipenem in rats with acute renal failure. Antimicrob. Agents Chemother. 45:3607
McWhinney B. C. ea. 2010. Analysis of 12 beta-lactam antibiotics in human plasma by HPLC with UV detection. J. Chromatogr. B Analyt. Technol. Biomed. Life Sc 878.:2039–43.
Lemaire-Hurtel A. S. ea. 2009. Recommended dosage adaptation based on renal function is not always sufficient to avoid betalactam antibiotics side effects. . Nephrol. Ther 5:5
Denooz R. CC. 2008. Simultaneous determination of five beta-lactam antibiotics (cefepime, ceftazidime, cefuroxime, meropenem and piperacillin) in human plasma by high-performance liquid chromatography with UV detection. . J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. 864:7
McWhinney B. C. ea. 2010. Analysis of 12 beta-lactam antibiotics in human plasma by HPLC with UV detection. J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. 878:5
Dailly E. BR, Deslandes G., Jolliet P., Le Floch R. 2011. 2011. A liquid chromatography assay for a quantification of doripenem, ertapenem, imipenem, meropenem concentrations in human plasma: application to a clinical pharmacokinetic study. . J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. 879:6
FDA. 2001. Guidance for industry, bioanalytical method validation. . FDA, Washington, DC.
R. C. 1997. Validation of chromatographic methods in biomedical analysis. Viewpoint and discussion.. J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. 689:6
Ratcliffe R. W. ea. 1989. Studies on the structures of imipenem, dehydropeptidase I-hydrolyzed imipenem, and related analogs. . J. Org. Chem. 54:653–60
Verdier M. C. TO, Tattevin P., Michelet C., Bentué-Ferrer D. Assessment of interindividual variability of plasma concentrations after administration of high doses of intravenous amoxicillin or cloxacillin in critically ill patients. J. Chemother.
Ratcliffe R. W. ea. Studies on the structures of imipenem, dehydropeptidase I-hydrolyzed imipenem, and related analogs. . J Org Chem1989.;54:653–60.
McWhinney B. C. ea. Analysis of 12 beta-lactam antibiotics in human plasma by HPLC with UV detection. J Chromatogr B Analyt Technol Biomed Life Sc. 2010;878.:2039–43.
How to Cite
This work is licensed under a Creative Commons Attribution-NoDerivatives 4.0 International License.