Antibiotic Susceptibility in metal tolerant Pseudomonas Spp. from Tannery waste water

Authors

  • Zameerul Hasan Department of Respiratory Medicine, King Georges Medical University, Lucknow, India
  • Seema Singh Department of Respiratory Medicine, King Georges Medical University, Lucknow, India
  • RAS Khuswaha Department of Respiratory Medicine, King Georges Medical University, Lucknow, India
  • Santosh Kumar Department of Respiratory Medicine, King Georges Medical University, Lucknow, India
  • Supriya Karpathak Department of Respiratory Medicine, King Georges Medical University, Lucknow, India
  • Rajeev Kumar Department of Respiratory Medicine, King Georges Medical University, Lucknow, India

DOI:

https://doi.org/10.21276/IJRDPL.2278-0238.2018.7(2).2954-2958

Keywords:

Heavy metal, Tannery effluent, Minimum Inhibitory Concentration, Pseudomonas spp

Abstract

In the present study total ten heavy metal resistant Pseudomonassp were isolated from Tannery waste water from Unnao (U.P) India, against Nickel, Chromium and Zinc. All the isolates exhibited high resistance to heavy metals with minimum inhibitory concentration (MIC) for heavy metals ranging from 100µg/ml to 1200µg/ml. All isolates showed multiple tolerances to heavy metal and were multi antibiotic resistant. Heavy Metal Tolerance Test indicated maximum microbial tolerance of Pseudomonassp (Ps-6&9) to Nickel (400 µg/ml) and no growth was found at 200 µg/ml to Zinc. All the Pseudomonas isolates were found to have a wide range of multi-drug and multi-metal resistant property indicating the tannery waste effluents are highly enriched which supports and spread of Antibiotics and heavy Metal tolerant microbes in the environment.

Downloads

Download data is not yet available.

References

Maitera, O.N., Ogugbuaja, V.A. and Barminas, J.T. Determination of trace metal levels in water and sediments of River Benue in Adamawa state, Nigeria. Journal of Ecology and the Natural Environment,2011; 3:149-156.

Unaldi Coral M N, Korkmaz H, Arikan B & Coral G.Plasmid mediated heavy metal resistances in Enterobacter spp. isolated from Sofulu landfill, in Adana, Turkey,2005; 55:175-179.

Cabrera, G., Viera, M., Gómez, J., Cantero, D. and Donati, E. Bacterial removal of chromium (VI) and (III) in a continuous system. Biodegradation, 2006; 18(4),505-513.

Issazadeh, K., Jahanpour, N., Pourghorbanali, F., Raeisi, G. and Faekhondeh, J. Heavy metals resistance by bacterial strains. Annals of Biological Research,2013; 4: 60-63.

Nasrazadani, A., Tahmourespour, A. and Hoodaji, M. Determination of Bacteria Resistance Threshold to Lead, Zinc and Cadmium in three Industrial Wastewater Samples. Journal of Environmental Studies,2011; 36:25-27.

Economou, V. and Gousia, P. Agriculture and food animals as a source of antimicrobial-resistant bacteria. Infection and Drug Resistance, 2015; 49.

Silver, S. Bacterial resistances to toxic metal ions-a review. Gene1996; 179: 9-19.

Vajiheh, K., Naser, B. and Giti, E. Antimicrobial, heavy metal resistance and plasmid profile of coliforms isolated from nosocomial infections in a hospital in Isfahan, Iran. African Journal of Biotechnology, 2003; 2(10),379-383.

Abdulla, D. Plasmids profiles, antibiotic and heavy metal resistance incidence of endophytic bacteria isolated from grapevine (Vitis vinifera L.). African Journal of Biotechnology,2009; 8(21), 5873-5882.

Bauer A.W, Kirby W.M, Sherris J.C, Turck M., (1966) Antibiotic susceptibility testing by a standardized single disk method. American Journal of Clinical Pathology, 45(4):493-6.

Abd-Elnaby, H., Abou-Elela, G.M. and El-Sersy, N.A. Cadmium resisting bacteria in Alexandria Eastern Harbor (Egypt) and optimization of cadmium bioaccumulation by Vibrio harveyi. African Journal of Biotechnology. 2011; 10: 3412-3423.

Oyetibo, G.O., Ilori, M.O., Adebusoye, S.A., Obayori, O.S., Amund, O.O.Bacteria with dual resistance to elevated concentrations of heavy metals and antibiotics in Nigerian contaminated systems. Environmental Monitoring and Assessment, 2010; 168:305-314.

Hussein, H., Moawad, H and Farag, S. Isolation and characterization of Pseudomonas resistant to heavy metals contaminants. Arab journal of Biotechnology,2004; 7: 13-22.

Samanta, A., Bera, P., Khatun, M., Sinha, C., Pal, P., Lalee, A. and Mandal, A. An investigation on heavy metal tolerance and antibiotic resistance properties of bacterial strain Bacillus sp. isolated from municipal waste. Journal of Microbiology and Biotechnology Research, 2012; 2: 178-189.

Virender S, Chauhan PK, Kanta R. Isolation and characterization of Pseudomonas resistant to heavy metals contaminants. Int J Pharm Sci Rev Res,2010; 3:164-167.

Ahemad, M., and Malik, A. Bioaccumulation of heavy metal by zinc resistant bacteria isolated from agricultural soils irrigated with wastewater. Bacteriology Journal, 20; 122:12-21.

Published

2018-04-15

How to Cite

Hasan, Z. ., Singh, S. ., Khuswaha, R. ., Kumar, . S., Karpathak, S. ., & Kumar, R. . (2018). Antibiotic Susceptibility in metal tolerant Pseudomonas Spp. from Tannery waste water. International Journal of Research and Development in Pharmacy & Life Sciences, 7(2), 2954 - 2958. https://doi.org/10.21276/IJRDPL.2278-0238.2018.7(2).2954-2958