A new analytical method development and validation for the estimation of Midodrine HCl by UV and HPLC

Authors

  • Vijeta Dhote Nri Institute of Pharmacy, Bhopal, India
  • Prabhat Jain Scan Research Laboratories, Bhopal, India
  • Vivek Jain Nri Institute of Pharmacy, Bhopal, India

DOI:

https://doi.org/10.21276/IJRDPL.2278-0238.2018.7(4).3060-3070

Keywords:

HPLC, UV, Midodrine hydrochloride, method development, Validation

Abstract

Two simple, accurate, sensitive and precise reverse phase-high performance liquid chromatographic and U.V. assay method for estimation of Midodrine hydrochloride (MD) was developed in tablet formulation. The chromatographic separation was performed on Thermo C18, (250 mm X 4.6 mm, 5 μm) column. The mobile phase consists of water: acetonitrile (30:70 v/v) pH adjust 3.5 with TEA was delivered at a flow rate of 1.0 ml/min and UV detection at 272 nm. The retention time of the drug was found to be 3.89 min. The developed method was found to be linear in a concentration range of 5-25μg/ml of the drug (r2= 0.999). The low value of % RSD indicates reproducibility of the methods. The low value of LOD and LOQ suggests the sensitivity of the method. Thus, this method can be used for routine analysis of Midodrine hydrochloride formulation and to check the stability of bulk samples.

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Published

2018-08-15

How to Cite

Dhote, V., Jain, P. ., & Jain, V. . (2018). A new analytical method development and validation for the estimation of Midodrine HCl by UV and HPLC. International Journal of Research and Development in Pharmacy & Life Sciences, 7(4), 3060 - 3070. https://doi.org/10.21276/IJRDPL.2278-0238.2018.7(4).3060-3070

Issue

Vol. 7 No. 4 (2018): Volume 7, Issue 4, July - August (2018)

Section

Original Article
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