Abstract
Two simple, accurate, sensitive and precise reverse phase-high performance liquid chromatographic and U.V. assay method for estimation of Midodrine hydrochloride (MD) was developed in tablet formulation. The chromatographic separation was performed on Thermo C18, (250 mm X 4.6 mm, 5 μm) column. The mobile phase consists of water: acetonitrile (30:70 v/v) pH adjust 3.5 with TEA was delivered at a flow rate of 1.0 ml/min and UV detection at 272 nm. The retention time of the drug was found to be 3.89 min. The developed method was found to be linear in a concentration range of 5-25μg/ml of the drug (r2= 0.999). The low value of % RSD indicates reproducibility of the methods. The low value of LOD and LOQ suggests the sensitivity of the method. Thus, this method can be used for routine analysis of Midodrine hydrochloride formulation and to check the stability of bulk samples.
References
Sethi PD, HPLC: Quantitative Analysis of Pharmaceutical Formulation; CBS Publishers and Distributors, New Delhi, 1996, 113-202.
Davidson AG, Beckett AH and Stenlake JB, Practical Pharmaceutical Chemistry; 4th edition; CBS Publishers and Distributors, New Delhi, 1989, 276-99.
United States Pharmacopoeia-35. National Formulary-30. Vol. 36. Pharmacopoeial forum; 2012. p. 414, 3922.
Drug bank. Available from: http://www.drugbank.ca/ drugs/DB00211.
Ghante MR, Tate AU, Development and validation of UV spectrophotometric methods for estimation of midodrine hydrochloride in bulk and tablet dosage forms, Inventi Rapid 2015,3,1-5.
Jain PS, Bhadane PV, Chaudhari HP, Surana SJ, Area under curve method development and validation of midodrine hydrochloride, Int J Pharm Chem Anal., 2015,2,154-60.
Jain HK, Gujar KN, Randhe VA, Stability indicating RP-HPLC assay method for estimation of midodrine hydrochloride in bulk and tablets. Int J Pharm Pharm Sci., 2016, 8(9), 283-287.
Jeffery G.H., Bassett J., Mendham J. and Denrey R.C.; Vogel's Textbook of Quantitative Chemical Analysis; 5th edition; Longman Group UK Ltd, England; 1989; 6-14.
Jain N., Jain R., Swami H. and Pandey S.; Indian Journal of Pharmacy and Pharmaceutical sciences,2009, 1(1), 189-191.
Khan, M.R. and Jain, D.; Indian Journal of Pharmaceutical sciences; 2006;64(6);546-548.
Meyer Veronica R.; Practical High-Performance Liquid Chromatography; 2nd edition, John wiley and sons, London;1993;56-58.
Hemant K. Jain, Kishor N. gujar, Varsha A. Randhe, Stability indicating RP-HPLC assay method for estimation of midodrine hydrochloride in bulk and tablets, International Journal of Pharmacy and Pharmaceutical Sciences, 2016; 8(9): 283-287.
S. Gopalakrishnan, K. Jothy and K. Dhanalakshmi, Analytical method development and validation of HPLC method for the determination of omeprazole in capsule dosage form, Elixir Appl. Chem. 52 (2012) 11283-11286.
Kalakonda Sri Nataraj, Mohammad BadrudDuza, Kalyani Pragallapati, Dussa Kiran Kumar, Development and validation of RP-HPLC method for the estimation of omeprazole in bulk and capsule dosage forms, International Current Pharmaceutical Journal 2012, 1(11): 366-369.
Hemant K. Jain, Kishor N. Gujar and Varsha A. Randhe. First order derivative spectrophotometric method development and validation for midodrine hydrochloride in bulk and tablets. World journal of pharmacy and pharmaceutical sciences.2016; Vol 5, Issue 7, 1760-67.
Singh Sunil, Choudhary Nisha, Rai Jyoti, Inamullah, Sharma Surabhi, Yadav Ajit Kumar, Gautam Hemendra, Chaturvedi Shashank, Agrawal Vipin Kumar, Validated RP-UPLC Method Development for Estimation of Lansoprazole in Tablet Dosage Form, International Journal of Pharmaceutical Sciences and Drug Research 2013; 5(3): 105-107.
Yarram Rama Koti Reddy, Sudershan Reddy G, M. R. P. Reddy and K. Mukkanti, Rapid simultaneous determination of aspirin and esomeprozole magnesium in combined tablets by validated ultra-performance liquid chromatographic method, Journal of Chemical and Pharmaceutical Research, 2013, 5(4):181-187.
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