Abstract
ABSTRACT: OBJECTIVES: To develop a simple, accurate, precise method was developed for the simultaneous estimation of the Sofosbuvir and Velpatasvir in tablet dosage form by Reverse Phase-High Performance Liquid Chromatographic Method (RP-HPLC). METHODS: Chromatogram was run through Standard Discovery C18 250 x 4.6 mm, 5?. Mobile phase containing 0.1% Orthophosphoric acid (Buffer with pH 3.2): Acetonitrile taken in the ratio 60:40 was pumped through column at a flow rate of 1 ml/min. Temperature was maintained at 30°C. Optimized wavelength selected was 240 nm. RESULTS: Retention time (RT) of Sofosbuvir and Velpatasvir were found to be 2.836 min and 3.678. %Relative Standard deviation (RSD) of the Sofosbuvir and Velpatasvir were found to be 0.4 and 0.2 respectively. %Recovery was obtained as 99.10% and 100.01% for Sofosbuvir and Velpatasvir respectively. Limit of detection (LOD), Limit of quantification (LOQ) values obtained from regression equations of Sofosbuvir and Velpatasvir were 0.10, 0.31 and 0.15, 0.46 respectively. CONCLUSIONS: Retention times were decreased and run time was decreased, hence, the suggested RP-HPLC method can be used for routine analysis of Sofosbuvir and Velpatasvir in Pharmaceutical dosage form.
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How to cite this article:
Sreehitha V, Sireesha R, Sivagami B, Kumar VP, Chandrasekar R, Babu MN. Simultaneous Estimation of Sofosbuvir and Velpatasvir Tablets by RP- HPLC Method Simultaneous Estimation of Sofosbuvir and Velpatasvir Tablets by RP- HPLC Method. Int. J. Res. Dev. Pharm. L. Sci. 2018; 7(5): 3092-3099. doi: 10.13040/IJRDPL.2278-0238.7(5).3092-3099.
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