Preparation and ex vivo evaluation of Microspheres for treatment of Urinary tract infections

Authors

  • K. Arshad Ahmed Khan Department of Pharmaceutics, Center for Pharmaceutical Research (CPR), Raghavendra Institute of Pharmaceutical Education and Research [RIPER], K.R. Palli cross, near S.K University, Anantapur, India
  • K. Rajesh Reddy Department of Pharmaceutics, Center for Pharmaceutical Research (CPR), Raghavendra Institute of Pharmaceutical Education and Research [RIPER], K.R. Palli cross, near S.K University, Anantapur, India
  • C. Haranath Department of Pharmaceutics, Center for Pharmaceutical Research (CPR), Raghavendra Institute of Pharmaceutical Education and Research [RIPER], K.R. Palli cross, near S.K University, Anantapur, India
  • C. Suryaprakash Reddy Department of Pharmaceutics, Center for Pharmaceutical Research (CPR), Raghavendra Institute of Pharmaceutical Education and Research [RIPER], K.R. Palli cross, near S.K University, Anantapur, India

DOI:

https://doi.org/10.21276/IJRDPL.2278-0238.2018.7(6).3164-3171

Keywords:

Biological Pigments, Green Chemistry, Phenolphthalein, Synthetic Indicators, Acid- base titrations

Abstract

Urinary tract infections (UTI) are the most frequent bacterial infections affecting about 150 million people each year. The most common cause of infections is E. Coli. UTI's are treated with short course antibiotics. Ofloxacin is a synthetic fluorinated carboxy quinolone that has a broad spectrum of activity against both gram positive & gram-negative bacteria. Multiple doses of ofloxacin are required to attain steady state concentration. The main objective of this study was to prepare and characterize ofloxacin microspheres to prolong the release rate so as to decrease the necessity of multiple dosing especially in patients with UTI. Nine formulations of ofloxacin microspheres were prepared using sodium alginate, sodium CMC and HPMC K 15M in various concentrations. Microspheres were prepared by ionic cross-linking method using calcium chloride as cross-linking agent. The prepared microspheres were evaluated for percentage yield, drug loading, entrapment efficiency, particle size analysis, swelling index, SEM studies, in-vitro dissolution, kinetics of drug release and ex-vivo studies. All the formulations exhibited satisfactory results in evaluations. DSC studies revealed no evidence of interaction between ofloxacin and polymers. Formulation F6 was selected as best formulation as it has good drug loading, percentage yield, entrapment efficiency and sustained the drug release for 8h. Results of ex-vivo studies indicate significant antimicrobial activity of microspheres. All the result demonstrated that ofloxacin microspheres can be effectively used in the treatment of urinary tract infections.

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References

Satoskar R.S, Bhandarkar S.D, Nirmala N. R. Pharmacology and Pharmacotherapeutics. Revised 24th ed. Popular Prakashan Pvt. Ltd (India): Elsevier India; 2015, 713-21.

George A & David C. Review of the Quinolone Family. In: Doughterty. TJ, Pucci MJ editors. Antibiotic Discovery and Development. 1st ed. Switzerland: Springer; 2012; 119-46.

Kadam N.R, Suvarna V. Microspheres: A brief review. Asian J Biomed Pharmaceut Sci. 2015; 5 (47): 13-9.

Rajesh kaza et al. Formulation and evaluation of Controlled release microspheres of Acyclovir sodium. International Journal of Innovate Pharmaceutical research. 2011; 2 (1): 98-101.

Poonampatii, Dakshachavanke, Milindwagh. A review on ionotropic gelation method: Novel approach for controlled gastro retentive gelispheres. Int J Pharm Pharm Sci. 2012; 4 (4):27-32.

Zein El. Deen E.E. Ghorab M.M, Shadeed Gad and Yassin H.A. Design and characterisation of diclofenac sodium microspheres prepared by ionotropic gelation technique for controlled drug delivery. International journal of Advances in Pharmacy, Biology and Chemistry. 2015; 4 (2): 321-329.

Koyel kar, Pal R.N and Bala N.N. Preparation, characterisation and evaluation of ropinirole hydrochloride loaded controlled release microspheres using solvent evaporation technique. Int J Pharm Pharm Sci. 2018; 10, 57-67.

Gulshan MD, Lakshmi Swapnasai M, Hemalatha T, Jhansisri U, Ramarao N. Formulation and development of microspheres for the treatment of familial adenomatous polyposis. Int J App Pharm. 2017; 9 (5):66-72.

Shruti Rathor and Alpana Ram. Porous microsphere of 5flouru uracil: a tool for site specific drug delivery in gastric cancer. Int J Curr Pharm Res. 2010; 3: 38-42.

Hindusthan Abdulahad, Pradeep Kumar B, Haranath C, Somasekhar Reddy K. Fabrication and evaluation of glimepiride cordial dichotoma G. Forst fruit mucilage sustained release matrix tablets. Int J Chem Sci. 2009; 7(4): 2555- 2560.

Bhargav E, Reddy CSP, Sowmya C, Haranath C, Khan KAA, Rajesh K and Srinath B. Formulation and optimization of piroxicam orodispersible tablets by central composite design. J Young Pharm. 2017; 9 (2):187-191.

Princely S, Saleem basha N, Nandhakumar S and Dhanaraju Md. Controlled delivery of antiretroviral drug-loaded cross-linked microspheres by ionic gelation method. Asian J Pharm Clin Res.2016: 9; 264-271.

Suvakanta Dash, Narasimha Murthy P, Lilakanta Nath and Prasanta Chowdhury. Kinetic modeling on drug release from controlled drug delivery systems. Acta Poloniae Pharmaceutica -Drug Research. 2010; 3(67): 217-223.

Shubha HS, Hiremath RS. Evaluation of antimicrobial activity of Rasaka Bhasma. Ayu. 2010; 31(2): 260-262.

Anuranjita Kundu1 and Sriparna Datta. Formulation and Characterization of Alginate Microbeads of Norfloxacin by Ionotropic Gelation Technique. International journal of Advances in Pharmacy, Biology and Chemistry. 2012; 1: 266-270.

How to cite this article:

Khan KAA, Reddy KR, Haranath C and Reddy CS. Preparation and ex vivo evaluation of Microspheres for treatment of Urinary tract infections. Int. J. Res. Dev. Pharm. L. Sci. 2018; 7(6): 3164-3171. doi: 10.13040/IJRDPL.2278-0238.7(6).3164-3171

This Journal is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported License.

Published

2018-12-15

How to Cite

Khan, K. A. A. ., Reddy, K. R. ., C. Haranath, & Reddy, C. S. . (2018). Preparation and ex vivo evaluation of Microspheres for treatment of Urinary tract infections. International Journal of Research and Development in Pharmacy & Life Sciences, 7(6), 3164 - 3171. https://doi.org/10.21276/IJRDPL.2278-0238.2018.7(6).3164-3171