Abstract
This paper have a purpose to determine the condition of analysis of betamethasone and dexchlorpheniramine maleat on tablet using ultraviolet spectrophotometry and high perfomance liquid chromatography (HPLC) methods. The spectrophotometry method used phosphate buffer pH 7,2 as the solvent, whereas the HPLC method used HPLC, LC-10AT VP, Shimadzu;µ BondapakTM C18 10µm 125Å, 4,6 x 150 mm coloumn Waters (Irlandia); methanol buffer (45:55) pH 7,2 as mobile phase; ultraviolet detection 240 nm; flow rate 1 mL/menit. Result showed that the correlation coefficient of spectrophotometry were 0,9998 and 0,9997 for dexchlorpheniramine maleat dan betamethasone at wavelength 239 and 262. The LOD for spectrophotometry were 2,261 ppm for dexchlorpheniramine maleat at λ 239 ; 0,707 ppm for dexchlorpheniramine maleat at λ 262 ; 0,088 ppm for betamethasone at λ 239 ; dan 0,127 for betamethasone at λ 262, the LOQ were 7,536 ppm for dexchlorpheniramine maleat at λ 239 ; 2,357 ppm for dexchlorpheniramine maleat at λ 262 ; 0,295 for betamethasone at λ 239 ; dan 0,425 for betamethasone at λ 262. The recovery percentation of the spectrophotometry methods for dexchlorpheniramine maleat and betamethasone were 101,32% and 100,77%. The recovery percentation of the HPLC methods for dexchlorpheniramine maleat and betamethasone were 107,6% and 100,8%. Coefficient of variance of the spectrophotometry methods methods for dexchlorpheniramine maleat and betamethasone were 1,413 % and 0,466 %, coeffisien of variance of the robustness test of the spectrophotometry methods for dexchlorpheniramine maleat and betamethasone were 0,834 % and 1,140 %. Based on this research has been found that the the analysis method of spectrophotometry was eligible for the validation parameter value. These data may be applied in Pharmaceutical industries.
References
Daru W (2013). Personal Communications, Pharmaceutical Industry PT. Sanbe Farma, Indonesia
deRuiter J (2001). Principles of drug action. http://www.auburn.edu/~deruija/hist_antihis.pdf (downloaded December 111th, 2013)
Hood DJ and Cheung HY (2003). A chromatographic method for rapid and simultaneous analysis of codeine phosphate, ephedrine HCl and chlorpheniramine maleate in cough-cold syrup formulation. J. Pharm. Biomed. Anal., 30:1595-1601
Farmakope Indonesia (FI ) Edisi IV (1994), Departemen Kesehatan Indonesia. Jakarta
Hugest DE (1998). Simultaneous determination of phenylephrine hydrochloride,
chlorpheniramine maleate and sodium benzoate by reversed-phase, pairedion and competing-base high-performance liquid chromatography, Journal of Chromatography A, 262(1983): 404-407.
Marin A, Garcia E, Garcia A and Barbas C (2002). Validation of a HPLC quantification of acetaminophen, phenylephrine and chlorpheniramine in pharmaceutical formulations: capsules and sachets. Journal of Pharmaceutical and Biomedical Analysis. 29: 701-714
Donato JL, Koizumi F, Pereira AS, Mendes GD, De Nucci G (2012) . Simultaneous determination of dextromethorphan, dextrorphan and doxylamine in human plasma by HPLC coupled to electrospray ionization tandem mass spectrometry: Application to a pharmacokinetic study. Journal of Chromatography. B (Print), v. 899, p. 46-56
Snyder LR and Kirkland J (1979). Introduction to Modern Liquid Chromatography. Second Edition. John Wiley & Sons.Inc NewYork, Chihester, Briebane, Toronto, Singapore
Tjay TH and Rahardja K., 2003, Obat-obat Penting, Edisi ke-5. Elekmedia Komputindo. Jakarta, pp 126.
Viana NS Jr, Moreira-Campos LM, Vianna-Soares CD (2005). Derivative ultraviolet spectrophotometric determination of dexchlorpheniramine maleate in tablets in presence of coloring agents, Farmaco, 60(11-12): 900-5.
Vignaduzzo SE and Kaufman TS (2013). Development and Validation of a HPLC
Method for The Simultaneous Determination of Bromohexine, Chlorpeniramine, Paracetamol, and Pseudoephedrine In Their Combined Cole Medicine Formulations, Journal of Liquid Chromatography & Related Technologies, 36:2829–2843
Weldesenbet DG (2008).Chemometrics-Assisted UV-Spectrophotometric determination of
betamethasone and dexchlorpheniramine maleate in laboratory prepared mixtures and combined tablet forms, Thesis, Addis Ababa University.
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