Abstract
A simple, specific, accurate, precise and sensitive Reverse Phase High Performance Liquid Chromatographic method has been developed for the quantitation of Tinidazole in both pure and pharmaceutical dosage forms. . Chromatographic separation achieved isocratically on a ODS2 reverse phase column [Use Symmetry C18, 250 X 4.6mm, 5µ] utilizing a mobile phase of Methanol. The flow rate was 0.5 ml/min and the effluents were monitored at 310 nm. The retention time was 4.610min. The linearity was in the range of 5-25µg / ml. This method was validated for linearity, precision and accuracy. Statistical analysis proves that the method is reproducible and selective for the estimation of the said drug.
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