Abstract
A simple, precise, rapid, selective, and economic reverse phase high performance liquid chromatographic (RP-HPLC) method has been developed for the simultaneous estimation of Lopinavir and Ritonavir in marketed formulations. Chromatographic separation was achieved isocratically for the combination was done with a C18 column [ ODS UG column. 250mm× 4.5 mm] utilizing mobile phase of composition Acetonitrile and Phosphate buffer (60:40v/v, pH 3) the flow rate was 1.5ml/min and the eluates was monitored at 220nm. Lopinavir and Ritonavir were eluted with retention times of 2.1min and 4.0min respectively. The method was found to be linear over a range of 20-100 g/ml for Lopinavir and Ritonavir. The method was validated according to the guidelines of International Conference on Harmonisation (ICH) and was successfully employed in the estimation of commercial formulations.
References
A.H. Beckett and J.B. Stenlake., “Practical Pharmaceutical Chemistry”,CBS Publishers and Distributors. Ed.4th, 2002.
D.A. Skoog, F.I. Holler and T.A. Nieman,“Fundamentals of Analytical Chemistry”, Saunders College Publishing, Ed.5th , 2005.
The Merck Index, “An Encyclopedia of Chemicals, Drugs andBiologicals”, Merck & Co, Inc, NJ, 2001.
Behera, Anindita, Moitra,(2011)Pharmacia Lettre, 3(1), 145-151.
Damaramadugu R, Inamadugu J, Kanneti R, (2010) Chromatographia, 71(9/10), 815-824.
Faux.J .Venisse. N, Olivier .J.Bouquet S, (2001)Chromatographia, 54, 469 473.
Myasein F, Kim E, Zhang J, Wu H, El-Shourbagy T, (2009)Anal Chim Acta 651, 112-116.
Seshachalam U, Haribabu B and Chandrasekhar KB, (2007)Biomedical Chromatography, 21(7), 716–723.
Suneetha A, Kathirvel S and Ramachandrika G, (2011)International journal of pharmacy and pharmaceutical sciences, 3(1), 49-50.
Usami Y, Tsuyoshi OK, Masahiko N, Sagisaka M, Kaneda T, (2003) Chem pharm Bull; 51,715-718.
This work is licensed under a Creative Commons Attribution-NoDerivatives 4.0 International License.
Copyright (c) 2020 Array