Abstract
The aim of the present study is to carry out the quantitative estimation of lupeol in poly herbal formulations by HPTLC and to study the invitro anti- inflammatory activity study of Crataeva nurvala bark. The bark of Crataeva nurvala was selected, dried, powdered and extracted with petroleum ether, diethyl amine and methanol successively by continuous hot percolation method using soxhlet apparatus. Preliminary phytochemical screening of the successive extracts showed the presence of chemical constituents like triterpenoids, flavonoids, alkaloids, tannins, glycosides and sesquiterpenes. The Rf value of lupeol was found to be 0.49 ± 0.03.The developed methods were validated as per ICH guidelines. The LOD and LOQ was found to be 50 and 300ng/spot respectively for HPTLC. The anti-inflammatory activity was highest in petroleum ether extract when compared to other extracts when evaluated by using ibuprofen as the control. This shows that the polyherbal formulation and medicinal plants containing lupeol as the active constituent has medicinal value and that they may be utillised for their anti- inflammatory properties.
References
Sethi PD. Quantitative Analysis Of Drugs in Pharmaceutical Formulations. 3rd ed. Mumbai: CBS Publishers and Distributors; 1997. p.1-56.
Nishritha. B, Sanjay. S Invitro regeneration of clonally uniform plants of crataeva magna: a high value medicinal tree by axillary branching method. New Forests. 2009;38(1)53-65.
Harwood L M, Moody C J. Experimental organic chemistry: Principles and Practice; Wiley Blackwell publishers; (Illustrated edition). p.122-125.
Agrawal S.S, Paridhavi. Herbal drug technology. P.324-326
Sanayaima RK, Kaur A, Agrawal A, Babbar SB. Cryopreservation of in vitro-grown shoot tips of Crateva nurvala Buch. Ham, an important medicinal tree. Cryoletters 2006;27(6):375-386
Walia N, Kaur A, Babbar SB. An efficient, in vitro cyclic production of shoots from adult trees of Crataeva nurvala Buch. Ham. Plant Cell Rep 2007;26(3):277-284
Martini LH, Jung F, Soares FA, Rotta LN, Vendite DA, Frizzo ME, et.al Naturally occurring compounds affect glutamatergic neurotransmission in rat brain. Neurochem Res 2007;32(11):1950-1956
Parimala S., Kuamr V.H., Kumar S.M.S., Suthakaran R., Selvan A.T., “Analgesic and CNS depressant Activity of Methanolic Extracts of Cissus Vitegenia And Cissus Pallida”, Int. J. Res. Dev. Pharm. L. Sci., 2012, 2 (2), pp. 330-332.
Kumari A, Kakkar P. Screening of antioxidant potential of selected barks of Indian medicinal plants by multiple invitro assays. Biomedical and Environmental Sciences 2008;21(1):24-29
Babbar SB, Walia N, Kaur A. Large scale invitro multiplication of Crataeva nurvala Methods Mol Biol 2009;547:61-70
Gagandeep, Meera, Kalidhar SB. Chemical Investigation of Crataeva nurvala Buch. Ham. Fruits.Indian Journal of Pharmaceutical science 2009;71(2)129-130
Pulok K.Mukherjee. Quality control of herbal drugs; second edition 2002; Business Horizons Pharmaceutical Publishers, P.405-406
Soxhlet, F. Die gewichtsanalytische Bestimmung des Milchfettes, Polytechnisches J. (Dingler's) 1879, 232, 461.
Agrawal S.A., “Evaluation of Anti Ulcer Activity of Oldenlandia Corymbosa (L)”, Int. J. Res. Dev. Pharm. L. Sci., 2012, 2 (2), pp. 363-367.
Pulok K.Mukherjee. Quality control of herbal drugs; second edition 2002; Business Horizons Pharmaceutical Publishers, chapter-13 HPTLC P.493-517
How to cite this article:
Jayaprakasam R., Velayutham S., jyothi B. A., Philips N., Ravi T. K., “Quantitative estimation of lupeol in polyherbal formulation and successive extracts of crataeva nurvala and their in vitro anti-inflammatory activity study”, Int. J. Res. Dev. Pharm. L. Sci., 2013, 2(6), pp. 699-704.
This work is licensed under a Creative Commons Attribution-NoDerivatives 4.0 International License.
Copyright (c) 2020 Array