Abstract
In this study, fenofibrate was examined both in vitro and in vitro. Its antioxidant activity in vitro was screened using DPPH assay; and specific antioxidant activity was observed using nitric oxide (NO•) assay, hydroxyl radical (•OH) assay and TBARS assay. The same substance was administered in mice to observe its antioxidant enzyme – GSH) and catalase - induction, inhibition of serum transaminases –SGPT and SGOT, and lipid lowering activities. It was observed in vitro that DPPH (IC50 > 0.38 mg/mL), NO• (IC50 = 22.81 µg/mL) and lipid peroxidation (IC50 = 30.37 mg/mL) was inhibited but not •OH (SC50 < 0.05 mg/mL). In vivo experimentation for lipid peroxidation showed that TBARS concentration was decreased by 24.79% while increasing concentrations of both GSH and catalase. Serum transaminases were reduced by 3.65% and 8.93%, respectively. Lipid profiling showed a decline in triglycerides and low density lipoproteins by 54.87% and 16.67%, correspondingly; while the amount of high density lipoproteins was augmented by 45.13%. Fenofibrate scavenges of free radicals, stimulates •OH production that may increase body’s defense against pathogens, boosts liver antioxidant enzyme against free radicals, and lowers serum transaminases. The results strongly suggest that fenofibrate, aside from its lipid-lowering activity may also provide antioxidant defenses.
References
Beltowski J, Wijcicka G, Mydlarczyk M, Jamroz A. (2006). The effect of peroxisome-activated receptors α (PPARα) agonist, Fenofibrate, on lipid peroxidation, total antioxidant capacity, and plasma peroxinase 1 (PON1) activity. Can J Physiol Pharm. 53 (3): 463-475.
Olukman M, Sezer ED, Ulker S, Sozmen EY, Cinar GM. (2010). Fenofibrate Treatment Enhances Antioxidant Status and Attenuates Endothelial Dysfunction in Streptozotocin-Induced Diabetic Rats. Exp Diabetes Res. Retrieved fromhttp://www.hindawi.com/journals/ edr/2010/828531/ last November 2011.
Watts GF, Staels B. (2006). Regulation of Endothelial Nitric Oxide Synthase by PPAR Agonists: Molecular and Clinical Perspectives. Atheroscler Thromb Vasc Biol. 24: 619-621.
Araki A. (2006). Homocysteine and diabetic macroangiopathy. Nippon Rinsho. 64(11):2153-8.
Mayer O, Simon J, Holubec L, Pikner R, Trefil L.(2006). Folate Co-administration Improves the Effectiveness of Fenofibrate to decrease the lipoprotein oxidation and endothelial dysfunction surrogates. Physiol Res. 55: 475- 481.
Chen L, Haught WH, Yang B, Saldeen TG, Parathasarathy S, Mehta JL. (1997). Preservation of endogenous Antioxidant activity and inhibition of lipid peroxidation as common mechanism of atherosclerotic effect of vitamin E, lovastatin and amlodipine. J Am Coll Cardoil. 30(2): 569-575.
Green RJ. (2004). Antioxidant activity of peanut plant tissues. Retrieved August 12, 2009 from the World WideWeb: http://www.lib.ncsu.edu/theses/available/etd- 11242004-075813/unrestricted/ etd.pdf
Winterbourn CC. (1995). Toxicity of iron and hydrogen peroxide: the fenton reaction. Toxicol Lett. 82(83): 969- 974.
Caillet S, Yu H, Lessard S, Lamoreux G, Ajdukovic D, Lacroix M. (2007). Fenton reaction applied for screening natural antioxidants. Food Chem. 100: 542-552.
Hazra B, Biswas S, Mandal N. (2008). Antioxidant and free radical scavenging activity of Spondias pinnata. J Altern Complement Med. 8(63): 1-10.
Sodipo OA, Abdulrahman F, Sandabe U. (2012). Effects of Aqueous fruit extract of Solanum macrocarpum Linn. On the haematological parameters of Chronic Triton- induced hyperlipidemic rats. J Phys Pharm Adv. 2(2): 122-132.
Keshetty V, Pabba S, Gudipatti R, Kandukuri JM, Allenki
V. (2009). Antihyperlipidemic activity of methnaolic extract of Garlic (Allium sativum L.) in Triton X-100- induced hyperlipidemic rats. J Pharm Res. 2(5), 777-780.
Oyinbo CA, Dare WN, Okugun GRA, Anyanwu LC. (2006). The hepatoprotective effect of vitamin C and E on hepatotoxicity induced by ethanol in sprague dawley rats. PJN. 5(6): 507-511.
Hakimoglu F, Kizil G, Kanay Z, Kizil, M, Isi H. (2007). The effect of ethanol extract of Hypericum lysimachioides on lipid profile in hypercholesterolemic rabbits and it’s in vitro antioxidant activity. Atherosclerosis. 192: 113–122.
Arnaiz LS, Travacio M, Monserrat AJ, Cutrin JC, Llesuy S, Boveris A. (1997). Chemiluminescence and antioxidant levels during peroxisome proliferation by fenofibrate. Biochim Biophys Acta. 1360(3): 222-228.
Kaur G, Jabbar Z, Athar M, Alam M. (2006). Punica granatum (pomegranate) flower extract possesses potent antioxidant activity and abrogates Fe-NTA induced hepatotoxicity in mice. Food Chem Toxicol. 44: 984-993.
Clifford DP, Repine JE. (1982). Hydrogen peroxide mediated killing of bacteria. Mol Cell Biochem. 49(3): 143-149.
Śliwa-Jóźwik A, Jóźwik A, Fronczyk W, Guszkiewicz A, Kołątaj A. (2004). Effect of reduced glutathione (GSH) on activity of lysosomal system in subcellular fractions of mouse kidney. Anim Sci Pap Rep. 22(2): 237-245.
El-Gendy KS, Aly NM. Mahmoud FH, Kenawy A, El- Sebae AKH. (2010). The role of vitamin C as antioxidant in protection of oxidative stress induced by imidacloprid. Food Chem Toxicol. 48: 215-221.
Halliwell F, Gutteridge J: “Free radicals in biology and medicine”, Oxford University Press , United States of America, Ed 4th, 2007.
Almo S, Smith DL, Danishefsky AT, Ringe D. (1994). The structural basis for the altered substrate specificity of the R292D active site mutant of aspartate aminotransferase from E. coli. Protein Eng. 7(3): 405–12.
Tsimihodimos V, Miltiadous G, Daskalopoulou S, Mikhailidis D, Elisaf M. (2005). Fenofibrate: Metabolic and pleiotropic effects. Curr Vasc Pharamcol. 3: 87-98.
Moberly J.B, Attman PO, Samuelesson O, Johansson AC, Knight-Gibson C, Alaupovic P. (1999). Apolipoprotein C- III, hypertriglyceridemia and triglyceride-rich lipoproteins in uremia. Miner Electrol Metab. 25(4-6): 258-562.
Keshetty V, Pabba S, Gudipatti R, Kandukuri JM, Allenki
V. (2009). Antihyperlipidemic activity of methnaolic extract of Garlic (Allium sativum L.) in Triton X-100- induced hyperlipidemic rats. J Pharm Res. 2(5), 777-780.
Oyinbo CA, Dare WN, Okugun GRA, Anyanwu LC. (2006). The hepatoprotective effect of vitamin C and E on hepatotoxicity induced by ethanol in sprague dawley rats. PJN. 5(6): 507-511.
Hakimoglu F, Kizil G, Kanay Z, Kizil, M, Isi H. (2007). The effect of ethanol extract of Hypericum lysimachioides on lipid profile in hypercholesterolemic rabbits and it’s in vitro antioxidant activity. Atherosclerosis. 192: 113–122.
Arnaiz LS, Travacio M, Monserrat AJ, Cutrin JC, Llesuy S, Boveris A. (1997). Chemiluminescence and antioxidant levels during peroxisome proliferation by fenofibrate. Biochim Biophys Acta. 1360(3): 222-228.
Kaur G, Jabbar Z, Athar M, Alam M. (2006). Punica granatum (pomegranate) flower extract possesses potent antioxidant activity and abrogates Fe-NTA induced hepatotoxicity in mice. Food Chem Toxicol. 44: 984-993.
Clifford DP, Repine JE. (1982). Hydrogen peroxide mediated killing of bacteria. Mol Cell Biochem. 49(3): 143-149.
Śliwa-Jóźwik A, Jóźwik A, Fronczyk W, Guszkiewicz A, Kołątaj A. (2004). Effect of reduced glutathione (GSH) on activity of lysosomal system in subcellular fractions of mouse kidney. Anim Sci Pap Rep. 22(2): 237-245.
El-Gendy KS, Aly NM. Mahmoud FH, Kenawy A, El- Sebae AKH. (2010). The role of vitamin C as antioxidant in protection of oxidative stress induced by imidacloprid. Food Chem Toxicol. 48: 215-221.
Halliwell F, Gutteridge J: “Free radicals in biology and medicine”, Oxford University Press , United States of America, Ed 4th, 2007.
Almo S, Smith DL, Danishefsky AT, Ringe D. (1994). The structural basis for the altered substrate specificity of the R292D active site mutant of aspartate aminotransferase from E. coli. Protein Eng. 7(3): 405–12.
Tsimihodimos V, Miltiadous G, Daskalopoulou S, Mikhailidis D, Elisaf M. (2005). Fenofibrate: Metabolic and pleiotropic effects. Curr Vasc Pharamcol. 3: 87-98.
Moberly J.B, Attman PO, Samuelesson O, Johansson AC, Knight-Gibson C, Alaupovic P. (1999). Apolipoprotein C- III, hypertriglyceridemia and triglyceride-rich lipoproteins in uremia. Miner Electrol Metab. 25(4-6): 258-562.
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