STUDY ON ISOLATION OF CYANOBACTERIA FROM JABALPUR REGION OF CENTRAL INDIA AND SCREENING THEIR PROTEASE INHIBITOR(S) ACTIVITY

Abstract

Central part of India is known for different environment throughout the year, in this way this part of the world has shown greater diversity among freshwater cyanobacteria. The present study showcases a screening program for the hunt of potent protease inhibitors compounds from cyanobacteria collected  from Central India. A total of 176 cyanobacterial samples collected, from different habitats of the Jabalpur area of Central India, which finally yielded 25 uni- algal cultures belonging to eight genera and all these genera belong to different species of cyanobacteria. These strains were grown in the laboratory for many generations and screened for their inhibitory activity against three major proteases trypsin, chymotrypsin and papain using an endpoint and kinetic assay. Extracts from Microcystis sp. DL09, Gloeocapsa sp. DL17 and Microcystis sp. DL22 were shown the most potential protease inhibitory activity against all three enzymes. During kinetic studies the IC50 values for all the three enzymes did not vary much from those observed during endpoint assays. The proposed research showed that these Cyanobacteria have showed promising protease inhibitor activities. In the context of the possible use of such protease inhibitors in pharmaceutics and other areas will be great potential for the future.