Abstract
Objectives: Thrombolytics are used in the management of thrombosis. They all work by activating the plasminogen into plasmin which dissolves the clots. But the major drawbacks with them are bleeding and anaphylactic reactions. Natural plants and their products with thrombolytic potential and being economical and safe can be considered as an alternative for cardiovascular diseases associated with thrombus. The purpose of the present study was to evaluate thrombolytic activity of a herbal mixture containing aqueous crude extracts of pericarp of Punica granatum, rhizome of Zingiber officinale and fruits of Phyllanthus emblica.
Methods: The In-Vitro clot lysis activity of 100µl of various proportions of herbal mixtures was studied by comparing with 100µl streptokinase as a positive control and 100µl of isotonic normal saline as negative control.
Results: In our study streptokinase was able to dissolve 68.5% of the clot. The aqueous extracts of Punica granatum, Zingiber officinale and Phyllanthus emblica on an average were able to dissolve 37.42%, 30.03% and 34.31% of the clot respectively. And the herbal mixture at 1:1:1 of Punica granatum, Zingiber officinale and Phyllanthus emblica was able to dissolve 40.19% of the clot.
Conclusions: From the present investigation it can be concluded that the herbal mixture possessed thrombolytic properties to some extent and can be a subject for further studies in this field.
References
Yuliana ND, Khatib A, Choi YH, Verpoorte R. (2011) Phytother.res. 25: 157-169
Bhaargavi V, Jyotsna GSL, Reshma T. (2014) IJPSR. 5: 690-702.
Katzung BG: “Basic and Clinical Pharmacology”, McGraw-Hill, New York, USA, Ed. 10th, 2007.
Shapiro SS. (2003) N Engl J. Med. 349: 1762–1764.
Califf RM, Topol EJ, George BS, Boswick JM, Abbottsmith C, Sigmon KN, et al. (1988) Am J. Med. 85: 353-362.
Alexandrov AV, Denchuk AM, Burgin WS, Robinson DJ, Grotta JC. (2004) J. Neuroimaging. 14: 113-117.
Sweta S, Choudhary GP. (2014) J. Pharmacogn Phytochem. 3: 194-197
Sampath R, Renuka S, Brindha P, Sivakumar R. (2016) Asian J. Pharm Clin Res. 9: 268-271.
Al- Rawahi AS, Edwards G, Al- Sibani M, Al-Thani G, Ahmed SH and Shafiur RM. (2014) EJMP. 4: 315-331
Debjit B, Gopinath H, Pragati kumar B, Duraivel S, Aravind G, Sampath kumar KP. (2012) J. Pharmacogn Phytochem. 1: 29-36
Shirin Adel PR, Jamuna P. (2010) J. Med. Plants Res. 4: 2674-2679
Samir M, Amrit pal S. (2003) Natural product radiance. 2(6): 296-300
Subash kumar G, Anand S. (2014) IOSR journal of pharmacy and biological sciences. 9: 124-128
Ekta S, Sheel S, Ashutosh P, Jaya D, Sachdev Y and Swapnil S. (2011) J. App Pharm Sci. 2: 176-183
Moazzem Hossen SM, Mohi U, Raihan MS. (2014) International journal of Pharmacognosy. 1: 307-316
Zia MU, Talha BE, Aninda Kumar N, Ismail MdH, Mohammad A, Sohel R. (2015) International Journal of Research in Pharmacy and Biosciences. 2: 1-9
Hamiduzzaman Md, Uttom K, Sultana JM. (2014) Int. Res. J. Pharm. 5: 151-154
Emanuele P, Paolo B, Serena MP, Ida M. (2011) Blood Transfus. 9: 120-138
Francois MB, Wensheng Pan MD, Hernan EG, Dale AP, Victor MDU, Kelly MB, Edlue MT. (2007) J. annepidem. 17: S24-S31
Md Arif D, Nahida T. (2012) International Current Pharmaceutical Journal. 1: 431-435
Nurtjahja TE, Ammit AJ, Roufogalis BD, Tran VH, Duke CC. (2003) Thromb Res. 111: 259-265
Koo K, Ammit A, Tran V, Duke C, Roufogalis B. (2001) Thromb Res. 103, 387-397
Marx W, McKavanagh D, McCarthy AL, Bird R, Ried K, Chan A, Isenring L (2015). PLoS ONE. 10(11), e0143675.http://doi.org/10.1371/journal.pone.0143 675
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