Abstract
Raphanus sativus L. is a most commonly used vegetable belonging to the family Cruciferae. In the present study, the plant material was procured and processed for cold extraction procedure using 70% ethanol. The extract was screened for the qualitative phytochemical analysis, in vitro lipid peroxidation inhibition and antiproliferative activites. From the results, Raphanus sativus L. revealed potent lipid peroxidation inhibition with an IC50 value 281.9 µg/mL compared to standard ascorbic acid and BHT. Further, MTT assay was used to measure cell proliferation against NIH-3T3, DLA and MCF7 cell lines and the significant results were recorded in the plant extract with an IC50 value of 415.4 µg/mL, 423.2 µg/mL and 703.5 µg/mL respectively.
References
Amador ML, Jimeno J, Paz-Ares L, Cortes-Funes H, Hidalgo M. (2003) Ann. Oncol. 14: 1607-1611.
Art IC, Hollman PC. (2005). Am. J. Clin. Nutr. 81: 317-325.
Cover CM, Hsieh SJ, Tran SH, Hallden G, Kim GS, Bjeldanes LF. (1998) J Biol Chem. 273:3838–47.
Czapski J. (2009) Vegetable Crops Research Bulletin. 70: 5-18.
Dillon A, Guidi AJ, Schnitt SJ:Diseases of the Breast", Lippincott Williams & Wilkins, Edition 4th, 2010.
George J., Todaro, Howard Green MD (1963) J Cell Biol, 17: 219-313.
Grever MCB: "Cancer drug discovery and development", Lippincott Raven, Philadelphia, 328-339, 2001.
Gulcin I, Mshvildadze V, Gepdiremen, A, Elias R. (2006) Phytomedicine, 13: 343.
Halliwell B, Guttridge JMC: "Free Radicals in Biology and Medicine" Japan Scientific Societies Press, Tokyo, Japan. Edition 2nd, 1989.
Jamuna KS, Ramesh CK, Riaz Mahmood, Pallavi M, Aditya Rao SJ. (2015) Int J Bioassays 4: 4653-4657.
Kaur C, Kapoor HC. (2001) Int J Food Sci Technol, 36: 703-725.
Khandelwal KR: "Practical Pharmacognosy techniques and experiments", Nirali Prakashan, Pune, Edition 16th,149-156, 2006.
Kim EK, Kim YS, Hwang JW, Lee JS, Moon SH, Jeon BT,et al. (2013) Process Biochem, 48:1086-90.
Kokate CK, Purohith AP, Gokhale SB: "Pharmacognosy", Nirali Prakashan, Pune, 120, 1990.
Li Y, Jiang B, Zhang T, Mu W, Liu J. (2008) Food Chem,106:444-50.
Mosmann T. (1983) J Immunol Methods, 65:55-63.
Nagy JA, Morgan ES, Herzberg KT, Manseau EJ, AM Dvorak, Dvorak HF. (1995) Cancer Res. 55:376–385.
Pandey BP: "A text book of Botany-Angiosperms: Taxonomy, Anatomy, Embryology and Economic Botany",S Chand and company Ltd. New Delhi,259-741, 2012.
Polterait O. (1997) Curr Org Chem, 1: 415-440.
Prior RL. (2003) American J Clin Nut, 78: 570S-578S.
Ramarathnam N, Osawa T, Ochi H, Kawakishi S. (1995) Trends in Food Sci Tech, 6: 75.
Saha MN, Alam MA, Aktar R, Jahangir R. (2008) Bangladesh J Pharmacol, 3: 90-96.
Scalbert A, Johnson IT, Saltmarsh M (2005). Am J Clin Nutr, 81: 215-217.
Trease GE, Evans WC: "A Text book of Pharmacognosy", Bailliere Tidall London, Edition 11th, 530, 1978.
Yuan JM, Gago-Dominguez M, Castelao JE, Hankin JH, Ross RK, Yu MC. (1998) Int J Cancer, 77: 211–6.
This work is licensed under a Creative Commons Attribution-NoDerivatives 4.0 International License.
Copyright (c) 2020 Array