Antioxidant properties of polyphenolic rich HPLC standardized extract of Beta vulgaris L. roots

Authors

  • Rania Indu Department of Pharmacology, R.G. Kar Medical College, Kshudiram Bose Sarani, Kolkata, India
  • Anjan Adhikari Department of Pharmacology, R.G. Kar Medical College, Kshudiram Bose Sarani, Kolkata, India
  • Moumita Ray Department of Pharmacology, R.G. Kar Medical College, Kshudiram Bose Sarani, Kolkata, India
  • Alok K. Hazra Chemistry Division, RKMA Quality Testing Laboratory (AYUSH), Narendrapur, South 24 Parganas, Kolkata, India
  • Tapas K. Sur Department of Pharmacology, Institute of Post Graduate Medical Education & Research, 244, A.J.C. Bose Road, Kolkata, India
  • Anup Kumar Das Department of Pharmacology, R.G. Kar Medical College, Kshudiram Bose Sarani, Kolkata, India

DOI:

https://doi.org/10.21276/IJRDPL.2278-0238.2017.6(3).2619-2624

Keywords:

Beta vulgaris, antioxidant, nitrite, superoxide, HPLC, protein denaturation

Abstract

OBJECTIVES: The present study was planned to evaluate the antioxidant effects of standardized Beta vulgaris L. or beetroot.

METHODS: The polyphenols present in lyophilized water extract of beetroot (BR) were quantified and standardized by HPLC. Deactivation of free radicals by BR was studied by in vitrobiochemical assays including 1,1-diphenyl-2-picrylhydrazyl (DPPH), hydrogen peroxide and superoxide radical scavenging activities. Nitrite content of BR was estimated. Moreover, inhibition of protein denaturation properties of BR was carried out.

RESULTS: HPLC chromatogram revealed presence of nine bioactive polyphenols in BR. Further, strong dose-dependent antioxidant properties were noted. IC50 (Inhibitory Concentration) of BR for DPPH, hydrogen peroxide and superoxide radical were 104.44µg/ml, 4.06µg/ml and 71.43µg/ml respectively. BR also showed enriched in nitrite content 3.359 mM/mg and it inhibited protein denaturation (IC50 594µg/ml).

CONCLUSION: Beetroot was found to possess strong antioxidant property that may be due to presence of polyphenols.

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Published

2017-05-15

How to Cite

Indu, R. ., Adhikari, A. ., Ray, M. ., Hazra, A. K. ., Sur, . T. K. ., & Das, A. K. . (2017). Antioxidant properties of polyphenolic rich HPLC standardized extract of Beta vulgaris L. roots. International Journal of Research and Development in Pharmacy & Life Sciences, 6(3), 2619-2624. https://doi.org/10.21276/IJRDPL.2278-0238.2017.6(3).2619-2624