BIODEGRADATION OF POLYTHENES BY BACTERIA ISOLATED FROM SOIL

Authors

  • Gauri Singh Department of Microbiology, Dolphin (PG) Institute of Biomedical and Natural Sciences, Manduwala, Dehradun (Uttarakhand) India.
  • Ashok Kumar Singh Department of Microbiology, Dolphin (PG) Institute of Biomedical and Natural Sciences, Manduwala, Dehradun (Uttarakhand) India.
  • Kalpana Bhatt Department of Microbiology, Dolphin (PG) Institute of Biomedical and Natural Sciences, Manduwala, Dehradun (Uttarakhand) India.

Keywords:

Consortium, Degradation, Environmental pollution and Polythene

Abstract

olythene plays an important role in packaging of goods, food material, medicine and garbage bags etc but its degradation is becoming a great threat and vital cause of environmental pollution. There are various polythene degradation methods available but the eco-friendly and acceptable method is by using microbes. The present study deals with the isolation, identification, screening and degradation of pretreated polythene by microorganisms obtained from soil. A total of 15 bacteria were recovered from different areas. Further Screening of polythene degrading microorganism was done by zone of clearance method out of 15 bacteria only 3 showed the positive results and identified to be Staphylococcus sp (P1A), Pseudomonas sp. (P1B), and Bacillus sp. (P1C). A total of three isolates P1A, P1B, PIC and one Consortium PID(P1A+P1B+P1C) were used for degradation of 10 and 40 micron polythene. Bacillus sp. (PIC) showed 42.5% followed by Staphylococcus sp. (P1A) 20% Pseudomonas sp. (P1B) 7.5 % and consortium (PID) 5% degradation by weight loss in 40 days. in 40 micron polythene. We can conclude that Bacillus sp may act as solution for the problem caused by polythene in nature. Hence from this study it can be speculated that microbes has enough potential to degrade plastic with due course of time.

Downloads

Download data is not yet available.

References

Augusta, J., R.J. Muller and H. Widdecke, (1993). A. rapid evaluation plate-test for the biodegradability of plastics. Appl. Microbiol. Biotechnol., 39: 673-678.

Bhardwaj H., Gupta R., Tiwari A. (2012), Microbial Population Associated With Plastic Degradation, Scientific Reports, 5: 272-274.

Cappuccino J.G., Sherman N., (1996), Microbiology- a laboratory manual, 159- 201.

El- Shafei, HA; El-Nasser, NHA; Kansoh, AL; Ali,AM (1998). Biodegradation of disposable of disposable polyethylene by fungi and Streptomyces species. Polymer Degradation and Stability ;62:361-365.

Frazer AC,(1994), O-methylation and other transformations of aromatic compounds byacetogenic bacteria. In: Drake HL, editor. Acetogenesis. New York:Chapman& Hall; p. 445-83.

Gnanavel G., Valli V. P. M. J., Thirumarimurugan M., Kannadasan T. (2012), Degradation of Plastics Using Microorganisms. Inter J of Pharmaceutical and Chemical Sciences, 1: 691-694.

Gu, J.D. (2003), Microbiological deterioration and degradation of synthetic polymeric materials. Res.Adv. Int. Biodeterior. Biodegrad. 52: 69-91.

Gu, J.D., Ford, T.E., Mitton, D.B. and Mitchell, R. (2000), Microbial degradation and deterioration of polymeric materials, W. Revie (Ed.), TheUhlig Corrosion Handbook (2nd Edition), Wiley, New York. 439–460.

Hamilton, J.D., Reinert, K.H., Hogan, J.V. and Lord, W.V. (1995), Polymers as solid waste in municipal landfills. J Air Waste Manage Assoc, 43: 247–251.

Hemashenpagam N, Growther L., Murgalatha N., Raj V. S.,Vimal S. S. (2013), Isolation and characterization of a bacterium that degrades PBSA. International Journal of Pharma and Bio Sciences, 4: 335–342.

Holt, J.G., N.R. Krieg, P.H.A. Sneath, J.T. Staley and S.T. Williams, (1994), Gram-Positive Cocci. In:Bergey's Manual of Determinative Microbiology, Hensyl, W.R. (Ed.). 9th Edn., Williams and Wilkins,

Jayasiri H. B., Purushothman C. S., VennilaA. (2013), Plastic litter accumulation on high-water strandline of urban beaches in Mumbai, India. Environ Monit Assess, 185: 7709-7719

Lee, B., Pometto, A.L., Fratzke, A. and Bailey Jr., T.B.(1991), Biodegradation of degradable plastic polyethylene by Phanerochaete and Streptomyces species. Appl. Environ.Microbiol. 57: 678-685.

Shimao M. (2001), Biodegradation of plastics. Current Opinion in Biotechnology ;12:242-247.

Sowmya,H.V., Ramalingappa.,Krishnappa, M. and B. Thippeswamy,(2014),Biodegradation of Polyethylene by Bacillus cereu,Advances in PolymerScienceand Technology: An International Journal,4(2): 28-32.

Starnecker, A; Menner, M (1996),Assessment of biodegradability of plastics under stimulated composting conditions in a laboratory test system.Int.Biodeterior.Biodegrad. 85 - 92.

Usha R., T. Sangeetha and M. Palaniswamy, (2011), Screening of Polyethylene Degrading Microorganisms from Garbage Soil,Libyan Agriculture Research Center Journal International 2 (4): 200-204

Vatsel D and Anbuselvi S, (2014), Isolation and Characterization of Polythene Degrading Bacteria from Polythene Dumped Garbage, Int. J. Pharm. Sci. Rev. Res., 25(2), Mar – Apr 2014; Article No. 38, Pages: 205-206

Yang H-S, Yoon J-S, Kim M-N.(2005) Dependence of biodegradability of plastics in compost on the shape of specimens. Polymer Degradation and Stability; 87:131-135.

Published

2016-03-15

How to Cite

Singh, G. ., Singh , A. K. ., & Bhatt, K. . (2016). BIODEGRADATION OF POLYTHENES BY BACTERIA ISOLATED FROM SOIL. International Journal of Research and Development in Pharmacy & Life Sciences, 5(2), 2056-2062. Retrieved from https://ijrdpl.com/index.php/ijrdpl/article/view/218