COMPARISON OF CONVENTIONAL PHENOTYPIC METHODS FOR DETECTION OF METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS
Keywords:
S. aureus , MRSA, MIC, Cefoxitin disc diffusion, oxacillin screen agar.Abstract
Background and Objectives: MRSA is probably the most challenging bacterial pathogen that currently affects patients in hospital and in the community. Hence Rapid and accurate detection of methicillin resistant Staphylococcus aureus (MRSA) is an important role of clinical microbiology laboratories to avoid treatment failure and to control the endemicity of MRSA. The aim of this study was to compare three conventional methods against the minimum inhibitory concentration (MIC) method to evaluate the best phenotypic method.
Materials and Methods: A total of 100 isolates of S. aureus were included in this study. Methicillin resistance was determined by oxacillin disc diffusion, cefoxitin disc diffusion the oxacillin screen agar test and MIC.
Results: Out of 100 isolates from our hospital, 29% and 36 % and 33% were identified as MRSA based on Cefoxitin disc diffusion, Oxacillin disc diffusion and Oxacillin screen agar test respectively. In all phenotypic methods, Cefoxitin disc diffusion test better correlates with gold standard method for detection of MRSA. Conclusion: - Our study revealed that cefoxitin disk diffusion method had a high sensitivity and specificity comparative to other phenotypic methods for detection MRSA.
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