EVALUATION OF ANTI-PROLIFERATIVE AND ANTIOXIDANT ACTIVITY OF SWERTIA CHIRATA: IMPLICATIONS IN BREAST CANCER

Authors

  • Homa Jilani Khan Department of Chemistry, Integral University, Lucknow, India, 226 026, Natural Products Research Lab, Department of Biochemistry, King George’s Medical University, Lucknow, India, 226 003.
  • Mohammad Kaleem Ahmad Natural Products Research Lab, Department of Biochemistry, King George’s Medical University, Lucknow, India, 226 003.
  • Abbas Ali Mahdi Natural Products Research Lab, Department of Biochemistry, King George’s Medical University, Lucknow, India, 226 003.
  • Namrata Rastogi Natural Products Research Lab, Department of Biochemistry, King George’s Medical University, Lucknow, India, 226 003.
  • Jamal Akhtar Ansari Department of Chemistry, Integral University, Lucknow, India, 226 026, Natural Products Research Lab, Department of Biochemistry, King George’s Medical University, Lucknow, India, 226 003.
  • Uzma Shahab Natural Products Research Lab, Department of Biochemistry, King George’s Medical University, Lucknow, India, 226 003.
  • Abdul Rahman Khan Department of Chemistry, Integral University, Lucknow, India, 226 026

Keywords:

SOD, Catalase, MTT, DPPH, Colony formation assay

Abstract

Swertia chirata belonging to Gentianaceae family is a medicinal plant known to exhibit various pharmacological properties.

This study investigates the antiproliferative effects of the aerial parts of S. chirata methanol extract (SCME) against breast cancer cells.

Antiproliferative effects of SCME were assessed on human breast cancer cell lines, MDA-MB-231 and MCF-7 using the MTT cell viability assay at concentrations ranging from 6.25 µg/mL to 400 µg/mL for 24 h, 48 h, and 72 h. Colony formation assay was performed to visualize the growth inhibition. Cellular apoptosis was determined by Hoechst staining. Additionally, enzymatic and non-enzymatic antioxidant activities were also assessed by DPPH, ABTS, TPC, SOD and CAT assays.

The results obtained indicate that SCME has potent cytotoxic activity towardsMDA-MB-231 and MCF-7 with anIC50value of 112 ± 4.5 μg/ mL and 180 ± 3 μg/ mL, respectively. Microscopic assessment of cellular and nuclear morphology of SMCE treated breast cancer cells indicated that SCME induced apoptosis as a mode of programmed cell death in these cells. Antioxidant profiling of SCME also indicated a high concentration of polyphenols and presence of antioxidant enzymes SOD and Catalase with potent free radical scavenging activity.

Our study demonstrated that SCME has significant antiproliferative activity against human breast cancer cells, MDA-MB-231 and MCF-7 and showed features of apoptotic cell death. Thus, S. chirata can be an enthusiastic target for researchers to exploit its potential as a natural treatment of breast cancer.

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Published

2016-01-15

How to Cite

Khan, H. J. ., Ahmad, M. K. ., Mahdi, A. A. ., Rastogi, N. ., Ansari, J. A. ., Shahab, U. ., & Khan, A. R. . (2016). EVALUATION OF ANTI-PROLIFERATIVE AND ANTIOXIDANT ACTIVITY OF SWERTIA CHIRATA: IMPLICATIONS IN BREAST CANCER. International Journal of Research and Development in Pharmacy & Life Sciences, 6(1), 2493-2501. Retrieved from https://ijrdpl.com/index.php/ijrdpl/article/view/178

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