Phytochemical analysis, exploration of antidiabetic and antioxidant potential of Anthocephalus cadamba (Roxb.)

Authors

  • Himanshu Pratap Singh Department of Pharmaceutical Sciences, Faculty of Health Sciences, Sam Higginbottom University of Agriculture, Technology and Sciences, Allahabad, India – 211007
  • Raghuveer Irchhaiya Department of Pharmaceutical Sciences, Faculty of Health Sciences, Sam Higginbottom University of Agriculture, Technology and Sciences, Allahabad, India – 211007
  • Amita Verma Department of Pharmaceutical Sciences, Faculty of Health Sciences, Sam Higginbottom University of Agriculture, Technology and Sciences, Allahabad, India – 211007
  • Himanshu Pandey Department of Pharmaceutical Sciences, Faculty of Health Sciences, Sam Higginbottom University of Agriculture, Technology and Sciences, Allahabad, India – 211007
  • Prem Prakash Singh Institute of Pharmacy, Bundelkhand University, Jhansi, India

DOI:

https://doi.org/10.21276/IJRDPL.2278-0238.2017.6(6).2800-2805

Keywords:

Antidiabetic, Antioxidant, methanolic extract, cadambine, Anthocephalus cadamba

Abstract

The most common disease nowadays is diabetes. In a fast-changing world, many means to treat diabetes naturally are explored by experts and clinicians today. Continuing use of insulin and other oral hypoglycemic agent creates undesirable side-effects, consequentially, unrestrained increase in blood sugar as well as complications associated with heart diseases; diabetics may have to face. To evade such glitches, herbal medications has superior advantages. As an alternative of using allopathic formulations, it is thus, gainful to use ayurvedic preparations for healthier regulation of diabetes mellitus. Current research was thus taken on to explore the anti-diabetic and antioxidant activities of crude extracts and pure compound obtained from the bark of Anthocephalus cadamba. The methanolic extract exhibited promising antidiabetic and antioxidant activity. Grounded on these results, the methanolic extract was fractionated on a silica gel column chromatography in a bioassay-led fractionation resulting in one known isolate, cadambine showing potent anti-diabetic activity, more than that of the positive control, glibenclamide. The results indicated that Anthocephalus cadamba methanolic extract (ACME) and the isolated compound (Cadambine) are potential natural agents to control diabetes. Alloxan significantly induced hyperglycemia; Oral administration of test samples for 14 days caused a significant decrease in blood glucose levels. The possible mechanism by which ACME mediated its antidiabetic effect could be by potentiation of pancreatic secretion of insulin from existing β-cells of islets in the extract treated animals.

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References

Davis, S. (2006). Insulin, oral hypoglycemic agents and the pharmacology of the endocrine pancreas. In: Brunton L, Lazo J, Parker K (eds) Goodman and Gilman’s, the pharmacological basis of therapeutics. McGraw Hill, New York.

Hunt, J.V., Dean, R.T., Wolff, S.P. (1988). Hydroxyl radical production and autoxidative glycosylation: glucose autoxidation as the cause of protein damage in the experimental glycation model of diabetes and aging. Biochem J. 256: 205–212.

Mukharjee, P.K. (2001). “Evaluation of Indian Traditional medicine”, J. Drug Information, 35: 631-640.

Tiwari, A., Rao, J. (2002) Diabetes mellitus and multiple therapeutic approaches of phytochemicals: present status and future prospects. Curr Sci 83:30–38.

Umachigi SP, Kumar GS, Jayaveera KN, Kishore DVK, Ashok Kumar CK, Dhanpal R 2007. Antimicrobial, wound healing and antioxidant activities of Anthocephalus cadamba. African J Tradit Complement Altern Med 4: 481-487.

Dr. Duke’s Phytochemical and Ethnobotanical Database 2010. ARS, National Genetic Resources Program. Germplasm Resources Information Network - (GRIN), National Germplasm Resources Laboratory, Beltsville, Maryland. Available on-line at http://www.ars-grin.gov/cgi-bin/duke/ethnobot.pl.

Chandrashekar KS, Abinash B, Prasanna KS. Anti-inflammatory effect of the methanol extract from Anthocephalus cadamba stem bark in animal models. Int J Plant Biol 2010; 1: 30-32.

Kapil A, Koul IB, Suri OP. Antihepatotoxic effects of chlorogenic acid from Anthocephalus cadamba. Phytother Res 1995; 9: 189-193.

Acharyya S, Rathore DS, Kumar HKS, Panda N. Screening of Anthocephalus cadamba (Roxb.) Miq. root for antimicrobial and anthelmintic activities. Int J Pharm Biomed Res 2011; 2: 297300.

Alam MA, Subhan N, Chowdhary SA, Awal MA, Mostofa M, Rashid MA, et al. Anthocephalus cadamba Ambujakshi HR, Antony ST, Kanchana Y, Riddhi P, Heena T, Shyamnanda. Analgesic activity of Anthocephalus cadamba leaf extract. JPR 2009; 2: 1279-1280.

Alam MA, Subhan N, Chowdhary SA, Awal MA, Mostofa M, Rashid MA, et al. Anthocephalus cadamba extract shows hypoglycaemic effect and eases oxidative stress in alloxan induced diabetic rats. Rev Bras Farmacogn 2011; 21: 155-164.

Shetti AA, Sanakal RD and Kaliwal BB. Antidiabetic effect of ethanolic leaf extract of Phyllanthus amara in alloxan induced diabetic mice. Asian J. Plant Sci. and Res. 2012; 2: 11-15.

Handa SS, Gupta SK, Vasisht K, Keene AT, Phillipson JD. Quinoline alkaloids from Anthocephalus chinensis. Planta Med 1984; 50: 358.

Sahu NP, Koike K, Jia Z, Achari B, Banerjee S, Nikaido T. Structures of two novel isomeric triterpenoid saponins from Anthocephalus cadamba. Magn. Reson Chem 1999; 37: 837-842.

Tramm N.L., Makotom S., Ryo, Y. (2006). Isolation and characterization of Celastrus rosmarinic acid oligomers in Celastrus hindsii Benth. leaves and their anti-oxidative activity. J Agric Food Chem 54:3786–3793.

Lemhadri A, Zeggwagh NA, Maghrani M, Jouad H, Michel JB, Eddouks M (2004) Hypoglycaemic effect of Calamintha officinalis Moench. in normal and streptozotocin-induced diabetic rats. J Pharm Pharmacol 56:795–799.

Trivedi NA et al., (2004) Effect of shilajit on blood glucose and lipid profile in alloxan induced diabetes rats. Indian J Pharmacol 36:373–376.

Ohkawa H, Oishi N, Yagi K (1979). Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 95.

How to cite this article:

Singh HP, Irchhaiya R, Verma A, Pandey H and Singh PP. Phytochemical analysis, exploration of antidiabetic and antioxidant potential of Anthocephalus cadamba (Roxb.). Int. J. Res. Dev. Pharm. L. Sci. 2017; 6(6): 2800-2805. Doi: 10.13040/IJRDPL.2278-0238.6(6). 2800-2805.

This Journal is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported License.

Published

2017-11-15

How to Cite

Singh, H. P. ., Irchhaiya, R., Verma, A. ., Pandey, H., & Singh, P. P. . (2017). Phytochemical analysis, exploration of antidiabetic and antioxidant potential of Anthocephalus cadamba (Roxb.). International Journal of Research and Development in Pharmacy & Life Sciences, 6(6), 2800 - 2805. https://doi.org/10.21276/IJRDPL.2278-0238.2017.6(6).2800-2805